Renoprotective Effect of Formononetin against Cyclophosphamide-Induced Oxidative Stress and Inflammation in Rat Kidney

Aims: Cyclophosphamide (CP) is a broad-spectrum chemotherapy agent available to treat various malignancies; however, its nephrotoxicity limits clinical use. Formononetin (FOR) bioactive isoflavone with encouraging biological activities. The current study explored and elucidated the possible protective/therapeutic effects of formononetin against CP-induced nephrotoxicity.
 Methodology: Rats received FOR (40 mg/kg/day) for 15 days followed by single injection CP on day 16. characterized an increase in urea creatinine levels serum. Kidney homogenate was used assess MDA, NO antioxidants.
 Results: CP-administered rats showed increased renal malondialdehyde nitric oxide along declined glutathione antioxidant enzymes. In addition, pro-inflammatory cytokines pro-apoptotic proteins decreased anti-apoptotic protein Bcl2 kidney. prevented kidney injury, enhanced antioxidants suppressed oxidative stress, mediators apoptosis.
 Conclusion: These findings suggest that prevents attenuating damage inflammation. Therefore, our data may represent novel protective strategy nephrotoxicity, which deserves pursuit further studies.